Today, the technological revolution has contributed to the development and advancement of biofertilizers, which have important benefits for the agricultural sector worldwide. This study aimed to analyze the barriers and components of the development of the use of biofertilizers and organic fertilizers in agricultural exploitation units of Nazarabad County, Alborz Province, using a descriptive-correlation method. The statistical population of the study was 2951 agricultural users in Nazarabad County, Alborz Province. The study villages and members of the statistical sample were selected using stratified sampling with proportional assignment and simple random sampling. To determine the sample size, Daniel's limited population correction formula was used, taking into account a 5% error rate; 228 people were determined. The main tool for data collection was a questionnaire. Confirmatory factor analysis was used to analyze the data. The results showed that infrastructure-technological barriers (0.830) are the most important barriers to the use of bio- and organic fertilizers in Nazarabad County, followed by economic-market barriers (0.780) in second priority, and socio-cultural and institutional barriers (0.699) in third priority. The results also showed that the economic-market mechanism (0.886) is the most important mechanism for the development of the use of bio- and organic fertilizers in Nazarabad County, followed by educational-promotional mechanism (0.841) in second priority, and socio-cultural and institutional mechanism (0.825) in third priority In order to develop the use of biological and organic fertilizers in Nazarabad County, the Agricultural Jihad Organization should provide appropriate educational and extension services according to the age and education of farmers.